home
***
CD-ROM
|
disk
|
FTP
|
other
***
search
/
Shareware Overload Trio 2
/
Shareware Overload Trio Volume 2 (Chestnut CD-ROM).ISO
/
dir26
/
med9410p.zip
/
M94A3280.TXT
< prev
next >
Wrap
Text File
|
1994-10-25
|
2KB
|
36 lines
Document 3280
DOCN M94A3280
TI AIDS pathogenesis: drug abuse, cytokines, and viral strain.
DT 9412
AU Xin K; Shapshak P; Nagano I; Stewart R; Dept. of Psychiatry, Univ. of
Miami Medical School, FL 33136.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):112 (abstract no. PA0065). Unique
Identifier : AIDSLINE ICA10/94369295
AB OBJECTIVE. We are investigating the pathogenesis of AIDS due to HIV-1
infection, drug abuse, and cytokines. METHODS. We utilize culture and
molecular techniques (in situ hybridization and immuno-histochemistry to
detect HIV and identify cell types in the central and peripheral nervous
systems) and PCR, cloning, and DNA sequencing to characterize the
sequence heterogeneity of HIV in blood, CSF, and brain. RESULTS. 1.
possible accelerative effects of cocaine, alcohol, and cocaethylene on
HIV replication in drug abusers; 2. not all cytokine production occurs
in HIV-infected cells in brain or sensory ganglia; 3. cocultures of
macrophages and astrocytes show accelerated HIV replication; 4. DNA
sequencing in the HIV envelope gene (V1-V5) is being done directly from
brain and phylogenetic relationships are being analyzed.
DISCUSSION/CONCLUSIONS. Specific envelope amino acid sequences may be
associated with neurological disease. Knowledge of the amino acid
sequence heterogeneity of HIV will contribute to the development of
vaccines against HIV.
DE Acquired Immunodeficiency Syndrome/COMPLICATIONS/*ETIOLOGY/
MICROBIOLOGY Astrocytes/MICROBIOLOGY Brain/MICROBIOLOGY Cells,
Cultured Cytokines/*PHYSIOLOGY DNA, Viral/GENETICS/ISOLATION & PURIF
Genes, env Human *HIV-1/GENETICS/ISOLATION & PURIF/PHYSIOLOGY
Macrophages/MICROBIOLOGY Substance Abuse/*COMPLICATIONS Support,
Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Virus Replication MEETING
ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).